Carlo Ambrosini was born in Azzano Mella (Lombardy) and graduated in painting from the Academy of Fine Arts of Brera in 1976. He made his debut in the world of comics in that same year, when he drew war stories for Dardo editors. Later he worked for Ediperiodici and Editoriale Corno, before contributing the graphic rendering for some episodes of the 'Storia d'Italia a fumetti' by Enzo Biagi. In 1980 he started contributing artwork to Sergio Bonelli Editore, illustrating seven 'Ken Parker' episodes. In 1984 he made the medieval series 'Nico Macchia', published in the magazine Orient Express. He also wrote some western scripts for Paolo Eleuteri-Serpieri, and worked as a press illustrator and advertising artist.
In 1986, he made 'Il Tesoro degli Imbala' with a script by Franco Mescola in Corto Maltese. For the French publisher Casterman and the magazine Comic Art, he created the character 'Barokko' two years later. Ambrosini also published in the Blue review and cooperated with the Phoenix publishing house. In Fumetti d'Italia, he made Videomax' with Graziano Origa in 1992. He was part of the art team of 'Dylan Dog' over at Bonelli, for which he illustrated a couple of episodes from 1987. Also at Bonelli, he created 'Napoleone', a character graphically inspired by Marlon Brando, in 1997.