Pierre Dupuis made his first story, 'Aigle Noir', in Gong in 1950. That same year, he was pushed by Paul Gillon into working for the publishing house Vaillant. He then made a story in the pocket book 34, before he joined the magazine Vaillant with several short stories in 1951. Still in 1950, he drew 'Au Pays des Ouèles' for Editions Mondiales and began a longtime collaboration with Chapelle, which lasted until 1970. He published 'Les Pirates de l'Infini', 'Anton Marcus', 'Alan Bruce' and 'Titan' in Zorro L'Invincible, 'Bison Noir' in Spécial Zorro and 'Cap 7', 'Erik le Viking' and 'Olac' in the publications of the same name.
Dupuis went to work for the World Press and Edifrance agencies in 1953, and drew several 'Belles Histoires de l'Oncle Paul' in Spirou, succeeded Albert Uderzo on 'Marco Polo' in La Libre Belgique and contributed to 'Les Grands Noms de l'Histoire de France' in Pistolin. In addition, he drew for France-Dimanche, Ici-Paris, La Semaine de Suzette and Fillette.
In 1960, he returned the Editions Mondiales, and collaborated with Hurrah! ( 'Robin des Bois', 'Jack Driant'), Télé Jeunes ('Télémaniac de Marcillac'), L'Intrépide, Intimité and Nous Deux. In 1964, he succeeded Raymond Poïvet on 'Mam'zelle Minouche', a series he continued with a script by Lécureux in L'Humanité until 1976.
In 1970, Pierre Dupuis was present in Hérisson and Marius, with most notably 'Pantzie'. He also drew 'Herminette' in 20 Ans and 'Albéric Barbier' in Spirou. He did short stories for La Vie Ourvrière, before he created 'L'Histoire de la Seconde Guerre Mondiale' for Hachette. In 1979 and 1980, he created 'Super Jaimie' in Téléjunior' and 'Kronos' in Albator. From 1980, he also cooperated with the 'Encylopédie en Bandes Dessinées' for the publishing house Auzou.
He went to work as an artist of historical comics for Dargaud, starting with 'L'Homme du 18 Juin - Charles de Gaulle' and followed by a great number of comic biographies in the collection Les Grands Capitaines. For these biographies, he mixed historical photographs with his drawings. In 1987, Dupuis made 'Les Fils du Dragon' with Jean Ollivier in Vécu. He also did several commission and educational albums. In the 1990s, he began 'L'Aventure Olympique' with Claude Moliterni and Thierry Roland at Dargaud, and took on a series of erotic comics for Média 1000. In the same register, he created 'Picabo' for Bédé Adult, starting in 1996.