Bo Hampton attended college at the School of Visual Arts in New York, where he studied under Will Eisner. He went to work as Eisner's assistant afterwards. In 1980 he started to work for Witching Hour. In 1994, Hampton completed the painted art for 'Batman: Castle of the Bat', and a vampire graphic novel entitled 'Verdilak' followed, based on a short story by A.K. Tolstoy. Later on, he pencilled the final issues of 'Batman and Robin Adventures', as well as the mini-series 'Batman Adventures: The Lost Year'.
In between, Hampton has done several works, varying from 'Swamp Thing' to 'New Mutants' and the graphic novel 'Legend of Sleepy Hollow'. He has also contributed to 'Confessions of a Cereal Eater' by Rob Maisch, 1995. He then spent a year as a professor at Savannah College of Art and Design, helping to develop the original incarnation of their Sequential Arts program before moving into the realms of storyboarding animated shows and TV commercials.