Born in Buenos Aires, Alberto Macagno started out drawing for scientific publications, as well as the advertising field. He entered the comics field through Alberto Breccia in 1967. His first job was at the publishing house Columba, where he took over the artwork of the series 'Hilario Corvalán' and where he illustrated comic adaptations of police, western and adventure films. With scenarist Robin Wood, he produced the series 'Gran Prix', 'Los Amigos' and 'Holbeck'. He had a longtime collaboration with the British Fleetway agency.
In the 1980s, Macagno worked for Record and illustrated several independent stories for La Urraca and Atlantida. In cooperation with writer Ricardo Ferrari and fellow artist Domingo Mandrafina, he produced two series at Editoriale Eura: 'Il Golem' and 'Diario di Bordo'. During his cooperation with Eura, he also drew several police and fantasy stories. For Bonelli, he illustrated some episodes of the series 'Julia' with Claudio Piccoli.