Gianluca Acciarino was born in Torre del Greco, a city situated in the Italian Province of Naples. In this city, he first studied accountancy but later graduated in political science. In 1998, he subsequently attended a course in comics at the Italian School of Comix.
He made drawings for two volumes of the series 'Eduardo a Fumetti' for the publisher Elledì '91. Since 2003 he collaborates with the Italian publisher Sergio Bonelli Editore, first on 'Brendon', and from 2011 on 'Tex Willer'. In 2012 he also collaborated with the French publisher Soleil as the artist of the third volume of the series 'Succubes'.