Born in Lyon, Didier Poli studied Applied Arts at the Emile Cohl School, as well as the CFT Gobelins. In the late 1990s, he started working for several animation studios, including Disney, Dupuis and Ubi Animation, and he became art director of Kalisto Entertainment in 2001. With writer Manuel Bichebois he created the fantasy comic 'L'Enfant de l'Orage', that was published by Les Humanoïdes Associés between 2003 and 2009.
Poli has since worked almost exclusively for Glénat, often in cooperation with other artists. He has worked with Jean-Baptiste Hostache on the artwork of such series as 'Neige Foundation' (2010-2012), 'Les Seigneurs de Guerre' (2012-) and 'Tenzing' (2013), and with Robin Recht on 'Elric' (since 2013). Poli and writer Guillaume Dorison were the driving forces behind the series of 'Petit Prince' adaptations, that also involved artwork by Moebius and a host of other artists.