Born in Florence, Pini Segna graduated from the Academy of Fine Arts in 1944. He settled in the Langhe area during the second World War and has lived there for the rest of his life. In 1946, he debuted with the Vincenzo Baggioli scripted 'Agente K6', followed by 'Il Giustiziere Mascherato', for the publishing house Nuova Aurora. In the following year, for Editoriale Sportiva, he made 'Penna Azzurra'. Starting in 1948, he worked as an illustrator of 'Saetta' and 'Volpe' stories for Alpe, and as a collaborator of Arc publications like 'Pantera Bionda', 'Tom Mix' and 'Tom Bill'.
In 1949, he began to work for the Albi dell'Intrepido collection. In the early 1950s, he drew series like 'Dix', 'Tornado Bill', 'Carabina Jones' and 'Gim Falco' for Marino Tomasina Editore, and in the late 1950s, he did art for 'Dick Montana', 'Gill Bart', 'Glory Men', 'Giungla Kid' and 'Dakota'. Between 1965 and 1977, he was mainly active as an illustrator of 'Mandrake', 'L'Uomo Mascherato' and 'Kriminal stories for Editoriale Corno, as well as 'Akim' for Editions Aventures et Voyages.
Between 1977 and 1982, he illustrated various stories with 'Zagor' for Daim Press. From 1990 to 2007 he was a collaborator of the local monthly Dogliani e le sue Langhe. He was in a car accident in the spring of 2007 and was hospitalized. A few weeks later he suffered a stroke and has since lived in a nursing home in Salmour. He passed away in September 2012.