Arne Zuidhoek studied at the Academy of Fine Arts in Amsterdam, and eventually settled in Utrecht. In 1968, he started Studio Wapper with Wim van de Sanden, through which he created comics for several local publications. The first was 'Bertus Knijp & Pieters' in Nieuw Utrechts Dagblad in 1968-1969, followed by 'De Vieze Nazels' in both Havenloods/Het Zuiden and Groot Utrecht in 1970-1971. Nieuw Utrechts Dagblad and Havenloods/Het Zuiden also ran his strip 'Wally' in 1969-1970.
He worked with Guus Dijkhuizen on 'Orgus' in Gandalf (1969-1970). Zuidhoek also published a comic in an art magazine and his comic 'Co-Starring Ko' appeared in the Dutch music magazine Aloha in 1969. He has written and illustrated many books, such as 'De Verzinsels van Vader Wapper' (1974), and text stories for Donald Duck.
Zuidhoek spent several years sailing with the merchant navy, and with his 63-foot schooner De Hoop. He is considered one of the leading publicists in maritime subjects, with an oeuvre of over thirty books. In the 1980s Zuidhoek moved to Santa Cruz, California, and decided to fully concentrate on his painting work, with a specialization in washed ink-drawing and orgrisaille techniques. One of the recurring themes in his paintings are pirates, and in 2002 there was an exhibition of his pirates in Slot Zeist.