Eugenio Colonnese was born in Italy, but has lived and worked in Brazil for most of his life. He began his career in Argentina in 1949. He started out working for El Tony, and eventually had a fruitful collaboration with the publishing house Columba. In 1964, he settled in Santo André, Brazil. Between 1964 and 1970, he was part of the Estúdio d'Arte, working next to artists like Rodolfo Zalla, Osvaldo Talo, Luiz Meri, Luscar and Rubens Cordeiro. During this period, Colonnese drew terror and war stories, and even worked with superheroes like 'Mylar', 'Superargo' and 'Escorpião'. For the terror magazines, he conceived characters like 'Mirza' and 'Morto do Pântano'. From 1970, Colonnese illustrated schoolbooks for Editora Ática, as well as educational comics. He became head of one of the artistic divisions of the publishing house in 1979, and remained at this position until 1999.
In 1980, he reassumed his comics activities, contributing to new terror magazines like Spectro, Calafrio and Mestres do Terror, by Editora D'Arte. At this time, he also drew the circus cowboy 'Beto Carrero'. Winner of several art prizes, and homaged by associations of artists and the governmental, he drew the history of Brazil for the Ministry of Foreign affairs in 1990. From 2001, he works for Opera Graphica, working on anthology albums, books with unpublished work and educational art books.