After having several jobs, Jacques Lob started making humorous illustrations in French magazines like Le Hérisson, Télé-Magazine, Fiction and Bizarre. In 1960 and 1961, he was present in Hara-Kiri. After Jean-Michel Charlier advised Lob to focus on writing scenarios and abandon drawing, he wrote several stories for artists like Pierre Guilmard, Mézières and Jo-El Azara, that were published in Pilote and Record. In 1964, he collaborated with Georges Pichard for the first time, creating the 'Ténébrax' series in Chouchou. After a brief stint in Tintin working with Azara, Lob teamed up with Morchoisne to create 'Kouakou' in the African magazine of the same name.
In 1966, he wrote two episodes of the western series 'Jerry Spring' for Jijé in Spirou. He subsequently teamed up with Pichard again to create 'Submerman' (1967, in Pilote), 'Blanche Épiphanie' (1967, in V Magazine) and 'Ulysse' (1968, in Linus and later Charlie Mensuel and Phénix). For Pilote magazine, he came up with 'Le Dossier Soucoupes Volantes', drawn by Robert Gigi that appeared from 1969 to 1975. From 1972, he wrote science fiction series for Philippe Druillet ('Lone Sloane') and José Bielsa ('Les Mange-Bitume'). That same year, he introduced the humorous superhero 'Superdupont' in Pilote together with Gotlib. After being drawn by Gotlib, 'Superdupont' was taken over by artists like Alexis and Jean Solé, and became one of the most popular series of Fluide Glacial from 1976.
In the mid-1970s, Lob took on drawing again, and created 'L'Homme au Landau' in L'Écho des Savanes, followed by 'Batmax' in 1981. He also launched the science fiction parody 'Roger Fringant' in Métal Hurlant (1976-79). With Alexis, he created 'Le Transperceneige', which remained unfinished for many years due to the artist's deaht in 1977. The story was eventually finished by Jean-Marc Rochette and published in À Suivre in 1982. Jacques Lob became the editor-in-chief of Chic magazine in 1984. In 1986, he wrote 'Intérieur Noir' for Edmond Baudoin in À Suivre, followed by 'Arlette et Charley' for Dan in Okapi. Also with Baudoin, he began the series about the female cab driver 'Carla' in 1988.